FACS vs MACS Which is better?

[noddyvinod]

HI Friends,

I am planning my experiments using CD4+ Human T cells. For which i need to purify them from the lymphocyte fraction isolated by the ficoll method from whole human blood.

What i want to know is which system do i choose.. FACS or MACS?

I want to transfect the purified cells for my experiment which means that a negative selection procedure is more apt for my experiment.

Please give me your suggestions and comments on this.

Kind Regards,

Vinod.

[ellesxbelles]

Hi Vinod,

It seems that you've answered the question for yourself really as you need your cells to be negatively selected. Personally I use the EasySep negative selection kit and I find that it works brilliantly, and I know that MACS do negative selection kits for CD4+ Human T cells. All you woulds then have to do is stain your cells with a conjugated CD4 antibody and run a small sample through your flow cytometer to check the purity of your population.

-elle