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FACS vs MACS Which is better?

FACS vs MACS Which is better? - Flow Cytometry Forum

FACS vs MACS Which is better? - Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.


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Old 12-24-2010, 09:47 AM
Pipette Filler
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Default FACS vs MACS Which is better?



HI Friends,

I am planning my experiments using CD4+ Human T cells. For which i need to purify them from the lymphocyte fraction isolated by the ficoll method from whole human blood.

What i want to know is which system do i choose.. FACS or MACS?

I want to transfect the purified cells for my experiment which means that a negative selection procedure is more apt for my experiment.

Please give me your suggestions and comments on this.

Kind Regards,

Vinod.
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Old 08-23-2011, 11:58 AM
Pipette Filler
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Default Re: FACS vs MACS Which is better?

Hi Vinod,

It seems that you've answered the question for yourself really as you need your cells to be negatively selected. Personally I use the EasySep negative selection kit and I find that it works brilliantly, and I know that MACS do negative selection kits for CD4+ Human T cells. All you woulds then have to do is stain your cells with a conjugated CD4 antibody and run a small sample through your flow cytometer to check the purity of your population.

-elle
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