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FACS vs MACS Which is better?
I am planning my experiments using CD4+ Human T cells. For which i need to purify them from the lymphocyte fraction isolated by the ficoll method from whole human blood.
What i want to know is which system do i choose.. FACS or MACS?
I want to transfect the purified cells for my experiment which means that a negative selection procedure is more apt for my experiment.
Please give me your suggestions and comments on this.
Re: FACS vs MACS Which is better?
It seems that you've answered the question for yourself really as you need your cells to be negatively selected. Personally I use the EasySep negative selection kit and I find that it works brilliantly, and I know that MACS do negative selection kits for CD4+ Human T cells. All you woulds then have to do is stain your cells with a conjugated CD4 antibody and run a small sample through your flow cytometer to check the purity of your population.
|The Following User Says Thank You to ellesxbelles For This Useful Post:|
|cd4+ , cell sorting , facs , macs|
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