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| Flow Cytometry Forum Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining. |
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#1
09-30-2010, 03:32 PM
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 Cells fixation Hi! Does somebody knows if cells can be stained after fixation with 2% paraphormaldeyde solution? Thanks!  |
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#2
09-30-2010, 06:05 PM
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 Re: Cells fixation Stained with what? We fix with 4% PFA and immunostain all the time. DNA stains should also work well. PFA disrupts membranes, so membrane stains may not work, or may work in a funky manner. Organellar-specific stains (i.e. lysotracker, mitoctracker) probably wont work, as most of them are attracted to the organelle by ion potentials or pH gradients - both of which are lost during fixation. Bryan |
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#3
09-30-2010, 11:28 PM
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| Re: Cells fixation We stain with 2% PFA and staining would be for see the membrane so probably won't work  Thank your very much for your reply. |
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| cells , facs , fixation |
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