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Scientist in crisis 09-30-2010 03:32 PM

Cells fixation
 
Hi!

Does somebody knows if cells can be stained after fixation with 2% paraphormaldeyde solution?

Thanks!

Warthaug 09-30-2010 06:05 PM

Re: Cells fixation
 
Stained with what?

We fix with 4% PFA and immunostain all the time. DNA stains should also work well. PFA disrupts membranes, so membrane stains may not work, or may work in a funky manner. Organellar-specific stains (i.e. lysotracker, mitoctracker) probably wont work, as most of them are attracted to the organelle by ion potentials or pH gradients - both of which are lost during fixation.

Bryan

Scientist in crisis 09-30-2010 11:28 PM

Re: Cells fixation
 
We stain with 2% PFA and staining would be for see the membrane so probably won't work :(

Thank your very much for your reply.


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