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Re: PBMC cell culture

Re: PBMC cell culture - Flow Cytometry Forum

Re: PBMC cell culture - Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.


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Old 08-24-2010, 11:29 PM
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Default Re: PBMC cell culture



I have solved my problem:

I wash my PHA stimulated PBMC in staining buffer (PBS + 5% FBS + 0.01% sodium azide). Set around 200,000 cells in 50 ul of staining buffer and stain with 5 ul of labeled antibody. RT for 20 min, a wash with staining buffer and finally fix with 1% paraformaldehide in PBS.

Cheers!
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