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appropriate fixation buffer for flow cytometric analysis of whole blood

appropriate fixation buffer for flow cytometric analysis of whole blood - Flow Cytometry Forum

appropriate fixation buffer for flow cytometric analysis of whole blood - Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.


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Old 08-21-2010, 11:31 AM
Pipette Filler
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Default appropriate fixation buffer for flow cytometric analysis of whole blood



Hello Everybody

Im going to do continues flow cytometric analysis of whole blood. my lab work will be heavy and i dnt want to loss any time. Im sure that i wil need to use fixative for some of my samples. I want to prepare a suitable fixative wiht long term shelf life. i know about paraformaldehyde but as i know its shelf life is about 3 - 4 monthes. can any one help me about a appropriate fixation buffer please?
thanks
Rozveh
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Old 08-23-2010, 10:08 PM
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Default Re: appropriate fixation buffer for flow cytometric analysis of whole blood

I use Paraformaldehide 1% diluted in PBS. Its preparation it's kind of time consuming, but the results are pretty good. Thanks for the shelf life info.
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