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PE/Cy7 Stickyness

PE/Cy7 Stickyness - Flow Cytometry Forum

PE/Cy7 Stickyness - Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.


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  #1  
Old 07-02-2010, 08:40 PM
Pipette Filler
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Exclamation PE/Cy7 Stickyness



Hello friends,

Our lab has recently been testing a series of antibodies with our flowcytometer. We have noticed that we get markedly different results when we use antibodies conjugated to PE/Cy7 fluorochrome compared with antibodies for the same target in another channel.

Does anyone else have this issue? Are we doing something wrong?



Steve
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Old 12-22-2010, 09:25 PM
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Default Re: PE/Cy7 Stickyness

How old is the PE-Cy7 antibody? Over time, the tandem dye can degrade (quicker than normal flours). You can tell if this is happening because it will start to spill over into the PE channel more then normal.
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