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#1
07-02-2010, 08:40 PM
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 PE/Cy7 Stickyness Hello friends, Our lab has recently been testing a series of antibodies with our flowcytometer. We have noticed that we get markedly different results when we use antibodies conjugated to PE/Cy7 fluorochrome compared with antibodies for the same target in another channel. Does anyone else have this issue? Are we doing something wrong?  Steve  |
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#2
12-22-2010, 08:25 PM
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 Re: PE/Cy7 Stickyness How old is the PE-Cy7 antibody? Over time, the tandem dye can degrade (quicker than normal flours). You can tell if this is happening because it will start to spill over into the PE channel more then normal. |
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| pe or cy7 , stickyness |
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