| | Re: variable cell counts for staining
I´m isolating my neuts and then aliquot equal amounts of PMN suspension for my different groups, so PMN numbers at the beginning of the experiment should be the same in each group (as I only compare the groups of one day=one neutrophil isolation, changes from day to day in absolute PMN numbers after isolation shouldn´t be a problem). Thing is that it is possible that, at the end of my experiments, there are differences in cell counts, although these differences shouldn´t be too big as all groups are handled the same way. But if, for example, I stain 1x10*6 PMN/ml with the same amount of mAB as 1,5x10*6 PMN/ml, would this change my FACS results?!