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|Flow Cytometry Forum Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.|
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I've infected some primary human mammary epithelial cells with an shRNA encoding lentivirus that bicistronically expresses GFP.
I'm labeling these cells with PE, APC, and PE-Cy5 conjugated antibodies. The APC and the PE-Cy5 look great, but the GFP signal is so intense that I can not possibly compensate enough to get a reliable and sensitive reading of the PE signal.
Anyone have any suggestions as to how I might ablate the GFP signal, but preserve the ability to detect the three cell-surface antigens?
|gfp , quench|