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Quench GFP?

Quench GFP? - Flow Cytometry Forum

Quench GFP? - Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.


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Old 06-30-2009, 10:00 PM
Pipette Filler
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Question Quench GFP?



I've infected some primary human mammary epithelial cells with an shRNA encoding lentivirus that bicistronically expresses GFP.

I'm labeling these cells with PE, APC, and PE-Cy5 conjugated antibodies. The APC and the PE-Cy5 look great, but the GFP signal is so intense that I can not possibly compensate enough to get a reliable and sensitive reading of the PE signal.

Anyone have any suggestions as to how I might ablate the GFP signal, but preserve the ability to detect the three cell-surface antigens?
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Old 10-04-2010, 11:24 PM
Pipette Filler
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Default Re: Quench GFP?

Confocal imaging.
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