I've infected some primary human mammary epithelial cells with an shRNA encoding lentivirus that bicistronically expresses GFP.
I'm labeling these cells with PE, APC, and PE-Cy5 conjugated antibodies. The APC and the PE-Cy5 look great, but the GFP signal is so intense that I can not possibly compensate enough to get a reliable and sensitive reading of the PE signal.
Anyone have any suggestions as to how I might ablate the GFP signal, but preserve the ability to detect the three cell-surface antigens?
Re: Quench GFP?
|All times are GMT. The time now is 09:13 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved