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Dissapearing epitope with RBC lysis

Dissapearing epitope with RBC lysis - Flow Cytometry Forum

Dissapearing epitope with RBC lysis - Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.


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Old 06-29-2009, 10:45 AM
Pipette Filler
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Default Dissapearing epitope with RBC lysis



Hi,

Has anyone had any experience of epitopes dissapearing with ammonium chloride red cell lysis. I am using human cells and know my epitope is not expressed on these cells (its expressed on CD45- CD235- marrow cells). I can detect it before RBC lysis but not after?

Anyhelp or suggestions would be gratefully appreciated.
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