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Flow cytometry analysis from fresh frozen tumor tissue samples

Flow cytometry analysis from fresh frozen tumor tissue samples - Flow Cytometry Forum

Flow cytometry analysis from fresh frozen tumor tissue samples - Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.


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Old 04-21-2009, 01:32 AM
Pipette Filler
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Default Flow cytometry analysis from fresh frozen tumor tissue samples



Does anyone have experience in doing flow cytometry analysis from frozen tumor tissue samples?? Which is the best protocol for frezeing down the tumor samples if you are interested in studying the expression of surface markers by flow cytometry later on: 1) cut the tumor into small pieces and then freeze down the tumor chunks immersed in FBS 10%DMSO solution; 2) digest the tumor and freeze down the single cell suspension in FBS 10%DMSO??

Thanks for your help in advance
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