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Isotype control - substraction or division
Hello to you all.
I encountered some difficulties running a flow cytometry analysis using the MFI values of the specific values against the isotype-matched control, as I haven't been able to find an unambigous answer as to whether the isotype value should be divided by or subtracted from the specific antibody value. I tried both methods, and while division yielded a very interesting and statistically significant pattern, substraction results an a highly-variant (nonsignificant) distribution of values.
Can anyone clarify which method - division or substraction - is correct to use?
|control , division , isotype , substraction|
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