| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Flow Cytometry Forum Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
03-16-2009, 04:58 PM
| |||||||||||
| |||||||||||
 Isotype control - substraction or division Hello to you all. I encountered some difficulties running a flow cytometry analysis using the MFI values of the specific values against the isotype-matched control, as I haven't been able to find an unambigous answer as to whether the isotype value should be divided by or subtracted from the specific antibody value. I tried both methods, and while division yielded a very interesting and statistically significant pattern, substraction results an a highly-variant (nonsignificant) distribution of values. Can anyone clarify which method - division or substraction - is correct to use? Thanks  |
|
| Tags |
| control , division , isotype , substraction |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| question on pcr product in control DNA | molbio123 | PCR - Polymerase Chain Reaction Forum | 2 | 05-04-2010 06:47 AM |
| negative control gives band same as positive control | adiza | PCR - Polymerase Chain Reaction Forum | 4 | 04-26-2010 06:03 PM |
| standard deviation when substraction and division | cathbr | General Science Questions and Layperson Board | 0 | 05-22-2009 09:30 AM |
| Control for 5'Aza treatment | hsinpai | Molecular Biology Techniques | 0 | 08-11-2008 02:24 AM |
| Photons Under Control | Ken Kubos | Physics Forum | 2 | 10-28-2004 11:54 PM |
Linear Mode
