Prevent Neutrophil Cells from Iatrogenic Activation:
Hi, Im currently analyzing neutrophils with flouroscences-conjugated anti-CD18/41/61 antibodies by BD Flow cytometer. However, the neutrophils from control mice are always activated!
I use 3.8% sodium citrate as anti-coagulate and 0.5%(v/v) paraformaldehyde for fixation.
I received a lot of positive anti-CD18/41/61 signals in control mice, especially anti-CD18 and my boss was mad and I was very sad
If anyone knows how to prevent iatrogenic activation of neutrophils, please please help me. Anything, any little tricks might help. Thank you very much.