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Invitrogen Click It proliferation assay

Invitrogen Click It proliferation assay - Flow Cytometry Forum

Invitrogen Click It proliferation assay - Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.


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Old 01-07-2009, 03:54 PM
Pipette Filler
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Default Invitrogen Click It proliferation assay



Has anyone had trouble with the Click It kit? I have used two separate kits and I'm following the protocol to the letter but still no luck. I have tried 10' 30' 45' and overnight labeling schemes on nicely proliferating diploid cells (great cell cycle profile) in suspension and seeded at 10e6/ml and I can't get it to work. I would appreciate information from others who have used this kit.
Thanks,
Kevin
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