|Register||Search||Today's Posts||Mark Forums Read|
|Flow Cytometry Forum Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.|
| ||LinkBack||Thread Tools||Display Modes|
how to use optimal antibody concentration
I want to analyze CD44 expression on peripheral CD8+ T cells from naive C57BL/6 mouse. but I found CD44 expression is heterogenous expression on CD8 T cells. Also, when I used saturated antibody to stain cells, the whole histogram peak moved to right with little overlap in isotype control. when I titrated down antibody concentration, the peak shifted to left. there is more overlap in isotype control. I do not know how to use correct antibody amount to stain the cells. In addition, how to define positive or negative expression? I feel that objective factors impact the data interpretation.
please help me to figure it out.
please reply email: [Only registered users see links. ]
I stained 10ul blood in 100ul volume.
|The Following User Says Thank You to leiset For This Useful Post:|
colleen agard (07-06-2010)
|antibody , concentration , optimal|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Custom antibody does not work as expected||jakekei||Antibody Forum||0||12-14-2009 09:53 PM|
|SUMO antibody problem (Nikola Wenta)||Suresh Kumar||Protocols and Methods Forum||0||05-19-2009 07:52 PM|
|Low concentration miniprep||Utsugi, Heidi K||Protocols and Methods Forum||0||01-15-2009 05:16 PM|
|Production of Monoclonal Antibodies - Technique in Mouse||Cellbiogal||Antibody Forum||2||01-08-2009 09:52 PM|