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I have problem in PBMC isolation

I have problem in PBMC isolation - Flow Cytometry Forum

I have problem in PBMC isolation - Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.


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Old 01-10-2008, 10:38 AM
Pipette Filler
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Question I have problem in PBMC isolation



I couldn't get nice images on flowcytometry when I isolate PBMC with Ficoll.Is it requisite for me to use NH4Cl to split Rbc?
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Old 08-16-2008, 06:23 PM
Pipette Filler
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Default Re: I have problem in PBMC isolation

Quote:
Originally Posted by photoshop1 View Post
I couldn't get nice images on flowcytometry when I isolate PBMC with Ficoll.Is it requisite for me to use NH4Cl to split Rbc?
hi, you can use ACK lysing buffer to lyse red blood cells and directly stain with antibody and run flow. It is not necessary to use ficoll to purify the lymphocytes.

try it
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Old 10-06-2011, 08:55 PM
Pipette Filler
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Default Re: I have problem in PBMC isolation

is it correct to do flowcytometry on pbmc population?
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