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I have problem in PBMC isolation

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Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.



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Old 01-10-2008, 09:38 AM
Pipette Filler
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Question I have problem in PBMC isolation

I couldn't get nice images on flowcytometry when I isolate PBMC with Ficoll.Is it requisite for me to use NH4Cl to split Rbc?
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