Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Flow Cytometry Forum
Register Search Today's Posts Mark Forums Read

Flow Cytometry Forum Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.

Intracellular Antibody Labelling

Intracellular Antibody Labelling - Flow Cytometry Forum

Intracellular Antibody Labelling - Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.

LinkBack Thread Tools Display Modes
Old 10-18-2007, 06:27 PM
Pipette Filler
Points: 1,117, Level: 19 Points: 1,117, Level: 19 Points: 1,117, Level: 19
Activity: 0% Activity: 0% Activity: 0%
Join Date: Oct 2007
Posts: 14
Thanks: 0
Thanked 0 Times in 0 Posts
Default Intracellular Antibody Labelling

Dear Fellow Researchers,

My colleagues and I ran into some hurdles trying to do intracellular staining for flow cytometry. We are trying to stain human astrocytes with fluorescent GFAP antibody, and analyze the cells on the FACScan. We do the fixation and permeabilization and then staining as suggested by the manufacturer, but are not seeing anything. Does anyone have any suggestions? I have heard that it's much harder to do suspension intracellular staining on fibroblast-body-type cells because they don't stretch out in suspension as well as on slides, which makes cytokeratine type staining difficult, is this true? Is there any special tricks to intracellular staining for cells in suspension? I'd appreciate any comment, thanks so much!

Reply With Quote

antibody , intracellular , labelling

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Custom antibody does not work as expected jakekei Antibody Forum 0 12-14-2009 10:53 PM
SUMO antibody problem (Nikola Wenta) Suresh Kumar Protocols and Methods Forum 0 05-19-2009 07:52 PM
Production of Monoclonal Antibodies - Technique in Mouse Cellbiogal Antibody Forum 2 01-08-2009 10:52 PM
primary vs secondary antibody public_enemee Western Blot Forum 1 10-31-2008 06:01 AM
[Zbrafish] Fw: ask for antibody houjia Zebrafish Forum 0 09-12-2007 04:22 AM

All times are GMT. The time now is 06:59 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11474 seconds with 16 queries