Hi,
i'm doing my research on plant model. So far we did not use any equal load control, the actin Ab we have in our laboratory doesn' work. So, i'm interested does anybody have had the same problem and solved it? What Ab did you use (tubulin or actin)

Regards,
manitas

Hello. I'm having a problem with proteins getting "stuck" at the top of the gel and not running correctly. This is a new problem in the past month. Previously the gels were running fine. The blue band runs normally to the bottom of the gel and the marker runs fine, but when I blot, the proteins are in a narrow smear toward the top of the gel. We make our own bis/acrylamide gels. Any suggestions?

hello everybody
i wanted to know if uneven or distorted wells could cause the bands to be distorted?:wiggle:

Hello,

1. Once the PAGE is prepared, how long can it be stored and under what conditions? For instance, if I prepare the gel on Friday afternoon, can I put the set gel in tank buffer and then store it in the fridge till Monday? Or would simply wrapping the gels in mQ H2O soaked paper towels work just as well?

2. I'm having trouble with our Santa Cruz alpha-tubulin antibody (SC-8035). It doesn't work well---its temperamental, sometimes it works and other times not and we don't know why. Anyone else having these problems? Found a solution? Alternatively, can one suggest another anti-house-keeping antibody which can be used as a loading control but also has multispecies activity. Ideally, I'm looking for something active against rat, goldfish and zebrafish. An alternative alpha-tubulin antibody would be most welcome.

p38 has been suggested and this would be great as my target proteins are all much larger than 38kDa but does it have multispecies activity?

Thanks

sample contains enzyme but in page bands are not clear