Molecular Biology Forum Life Science Forums - Flow Cytometry Forum http://www.molecularstation.com/forum/ Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining. en Mon, 23 Nov 2009 22:45:23 GMT vBulletin 60 http://www.molecularstation.com/forum/images/misc/rss.jpg Molecular Biology Forum Life Science Forums - Flow Cytometry Forum http://www.molecularstation.com/forum/ Autofluoresent macrophages http://www.molecularstation.com/forum/flow-cytometry-forum/71876-autofluoresent-macrophages.html Thu, 19 Nov 2009 22:02:08 GMT Are all murine macrophages autofluoresent? or do they differ depending on tissue?
Are all murine macrophages autofluoresent?
or do they differ depending on tissue?
]]> Flow Cytometry Forum hes http://www.molecularstation.com/forum/flow-cytometry-forum/71876-autofluoresent-macrophages.html variable cell counts for staining http://www.molecularstation.com/forum/flow-cytometry-forum/71872-variable-cell-counts-staining.html Thu, 19 Nov 2009 12:23:07 GMT Dear all, I am using FACS to evaluate receptor expression on PMN. For each experiment, I got different groups (they only differ in the volatile...
Dear all,

I am using FACS to evaluate receptor expression on PMN. For each experiment, I got different groups (they only differ in the volatile anesthetic they are incubated with, the rest of the procedure is standardised and remains the same for all PMN). I therefore assume that there should not be any significant changes in cell count between groups. Regarding the AB-staining, would changes in PMN-counts (say +/- 30%) be a problem for further inter-group analysis? PMN are washed after staining...

Thanks
]]> Flow Cytometry Forum brettsportler http://www.molecularstation.com/forum/flow-cytometry-forum/71872-variable-cell-counts-staining.html Quantification?! http://www.molecularstation.com/forum/flow-cytometry-forum/71097-quantification.html Wed, 04 Nov 2009 15:25:17 GMT Dear all, how do you quantify or standardise your FACS results? I know there are several forms of quantification beads, e.g. Quantibrites from BD...
Dear all,

how do you quantify or standardise your FACS results? I know there are several forms of quantification beads, e.g. Quantibrites from BD for this purpose. But as I am using the same FACS-machine all the times and make sure that the voltage for the channel I´m looking at is constant, e.g. 600 for APC-Cy7, do I really need this???

Thanks for your help, cheers

Björn
]]> Flow Cytometry Forum brettsportler http://www.molecularstation.com/forum/flow-cytometry-forum/71097-quantification.html flow cytometry help with scattergram http://www.molecularstation.com/forum/flow-cytometry-forum/70760-flow-cytometry-help-scattergram.html Sun, 27 Sep 2009 22:31:40 GMT Any help with a diagnosis would be appreciated. Thanks. See attached scattergram picture.
Any help with a diagnosis would be appreciated. Thanks.
See attached scattergram picture.

Attached Images
File Type: jpg Flow_Cyto_Pic.jpg (94.4 KB)
]]> Flow Cytometry Forum pathstudent http://www.molecularstation.com/forum/flow-cytometry-forum/70760-flow-cytometry-help-scattergram.html