Could someone please tell me a recipe for making phosphate buffered saline 0.1M pH7.4.

Thanks!

Hallo,
im using precast anamed 10% , 15%TBE UREA PAA gels. the maximum length of ssdna in my sample is 80 bases. I have a oligostandard 20 to 100 bases from IDT. the protocol says i should use a Voltage 180 v and current 30 mA, but i cant get both of them together. i have a current of 75 mA for 180 V. I use 1 X TBE as running buffer. should i dilute it to 0.5 to reduce ion concentration also the protocol says max 70 min run. even after 4 hrs i dont get seperation of ladder. if i do overnight i get bands in sample many bands due to fragmentation i think, but ladders goes out of gel.
please suggest some remedy to this problem. i use Loading buffer from biorad for denaturing gels.
Thanks for suggestions,
Regards,
Harish.

Hello everyone,

I was just wondering how long samples can be kept frozen at -80C before degradation? They are in 100 ul lysis buffer with 1mM leupeptin and and 1mM aprotinin. Thanks for your help.

Hi friends,

My problem looks so simple but it is complicating my experiments. I tried to isolate proteins from human adipose tissue but I ended up having red colour(coming from blood vessels/red blood cells) in my lysate which I cannot get rid off. This red color is interfering with the protein concentration measurement. My supervisor is asking me for the results and the time is very limited. Pleaaaaaase help me with any replies how to isolate these cells or blood vessels from adipose cells.
Thanks.