There's a new kit from Invitrogen that makes antibody crosslinking a thing of the past. It covalently couples the antibodies to the beads and it works great.

It's called the Dynabeads Antibody Coupling Kit... give it a try :)

it's magnetic bead based so if you're not into magnetic beads then this may not be for you

Hi,
I am looking for labs (be it academic or biotech) who would be interested in commercializing their antibodies.We over 24 years of experience in providing diagnostic industry and research community with quality antigens and we would like to collaborate with your lab if you produce antibodies.
Looking forward to hearing from you.
AntibodyGeek

Please let me know which antibody is suitable to stain cultured glutamatergic neurons.

I want to see whether the neunons are glutamatergic neurons.

Thanks,

immunologist

How do we stain peripheral sensory neuron and peripheral motor neuron?

Which antibodies to use?

Thanks,

immunologist

Hello everyone, I am looking for primary antibodies for immunohistochemistry on PFA-perfused, cryoprotected frozen sections.
A fair few of the antibodies list different applications like Western Blotting, ELISAs, Immunoprecipitation and not Immunohistochemistry.

I feel really confused and out of my league, and do not understand why some of these antibodies cannot be used for an immunohistochemistry and immunocytochemistry? Are they conjugated to something else?!

Thank you for helping me clarify this....