Molecular Biology Forum Life Science Forums - Instructions for Posting, Help, and Frequently Asked Questions http://www.molecularstation.com/forum/ en Mon, 23 Nov 2009 22:29:03 GMT vBulletin 60 http://www.molecularstation.com/forum/images/misc/rss.jpg Molecular Biology Forum Life Science Forums - Instructions for Posting, Help, and Frequently Asked Questions http://www.molecularstation.com/forum/ Y2H Identification of Prey Protein http://www.molecularstation.com/forum/instructions-posting-help-frequently-asked-questions/70554-y2h-identification-prey-protein.html Wed, 09 Sep 2009 09:32:06 GMT Hi all, It is my first post. Stuck at yeast two hybrid system. Screening is done. Unable to identify the target protein. Isolated yeast plasmid DNA...
Hi all,
It is my first post. Stuck at yeast two hybrid system. Screening is done. Unable to identify the target protein. Isolated yeast plasmid DNA , electroporated in E. coli ans selected for Amp resistance (prey vector). Some colony appeared. Purified the DNA from E. coli BUTTTTT the digestion profile does not give the desired match.
Can anyone suggest something.
Ur kind help is required.
Mamoon
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