Could any one explain how northern blotting can be used to analyse gene expression, and how extraction of RNA (which is what you did) is an essential prerequisite to northern blotting, Please ?

Hello biochemists,

Could any one explain how northern blotting can be used to analyse gene expression, Please ?

Actually, I did RNA extraction from plant last week. all extractions showed optical absorbance readings, yet some of the samples which
showed optical absorbance readings did not show any bands on the gel. Why might this be?

Attach Files
1-photo taken 2 hrs after the gel was loaded
2- photo taken after the curent and voltage was reduced and the gel run overnight

Attached Images

	photo taken 2 hrs after the gel was loaded EX3&4.jpg (99.2 KB)
	photo taken after the curent and voltage was reduced and the gel run overnight..jpg (98.6 KB)

Hello to you all!
My lab started to work with syber safe, as a replacement for EtBr, we are adding syber directly into agarose gel, same as we did with EtBr...but we have a problem with visualization of bends, they are starting to fade after 10-15 sec...it is realy frustratig! Has any one had similar problem?

Thanks!

Hey guys~~~
I was trying to use 3% agar gel for distinguishing the DNA products which vary in sizes from 5bp to 300bp while it seems to be a failure...
I am not sure whether it is fine using the 3% agar gel or should I use the polyacrylamide gel?

looooook forward to all your help and thank you in advance!

liv

Hi, I'm using a 4% gel to run 8 samples that vary by buffer and 2 ladders (25 and 100bp) at 130 volts for 30 min.

My question is: what would cause a lack of bands to show up? I've tried twice and I still have nothing. Someone else working in my lab got bands to show, so it cant be EtBd or any other products used in the process. Does anyone know of any common errors that might make a gel not run properly? I'm wondering if I'm making my gel wrong, but it doesn't appear odd.

I'm not expecting anyone to read this and be able to post something like "This is definitely what you're doing wrong..." but I'm an undergrad student and I'd really like to walk in on Tuesday with a good idea of what I may need to change to make my gel work.