Im planning to do qPCR on E. coli K12.
I read an article which stated that dxs gene was used as housekeeping gene in E. coli. Alkaline phosphate (phoS), beta-D-glucuronidase (uidA) or acid phosphatase (phoA) can be used as housekeeping gene?
May I know any others housekeeping gene that i can use?

Hi All,

we want to perform bioburden testing of cefuroxime. we have found a millipore paper giving that penicillinase to be added to neutralise the antibiotic before testing bioburden but the conc is mentioned in mg and in vial it is in units can anyone help me out with this.

thanks

Hey guys, I'm new to the forum so bear with me...

I've got this last question that I cannot find an answer to, here it is:

The incubation of (NAG)4 with lysozyme results in the slow formation of (NAG)6 and (NAG)2. Propose a mechanism for this reaction.

Could someone guide me in the right direction? or link me to an answer?

Thanks in advance:)

Are all murine macrophages autofluoresent?
or do they differ depending on tissue?

Previous work by K. Prasadan showed Morpholinos enter rodent embryonic pancreatic cells from the extracellular space. Several papers from University Paris Diderot-CNRS show uptake into the pancreas of rat pups after s.c. injection of bare or fluor-tagged Morpholinos:
Uzan B, Figeac F, Portha B, Movassat J. Mechanisms of KGF Mediated Signaling in Pancreatic Duct Cell Proliferation and Differentiation. PLoS ONE. 2009;4(3):e4734. Epub 2009 Mar 6.
Figeac F, Uzan B, Faro M, Chelali N, Portha B, Movassat J. Neonatal growth and regeneration of {beta} cells are regulated by the Wnt/{beta}-catenin signaling in normal and diabetic rats. Am J Physiol Endocrinol Metab. 2009 Nov 17. [Epub ahead of print]
While we at Gene Tools are habitually skeptical of claims of delivery of bare Morpholinos dosed systemically in vivo, this is a case where previous careful work has shown uptake of bare oligos into embryonic cells of this type. We anticipate further work in this system with keen interest.