For anyone doing cryopreservation, check out searchable, browsable directory of protocols for cryopreservation. Cryosearch has hundreds of protocols for the cryopreservation of many different cell and tissue types from a wide variety of species.

Why was Cryosearch created? Because cryopreservation is done in a million different ways and there's little or no consensus on what the best method is for any given sample. We're hoping that be developing this platform we can start to fix that. (This is not some commercial gimmick. It's free to use and there are no ads.)

You can also upload your own protocols to help build the protocol database and comment and upvote / downvote protocols to help others find the protocols that work best for everyone.

Hi,
I am doing "spring-cleaning" :-) in the lab and it seems to be probably the first cleaning in last 10 years. :-( I found huge secred reserve of HAT MEDIA Supplement (Sigma Aldrich, #H-0262, Lot 24H9414) in our freezers. The supplement is lyophilized and stored at -20*C for at least 5 years, but rather longer (I was not working here that time so can not say exactly). The current field of interest of the lab is quite different and we can not use the HAT MEDIA supplement. Thus I need to know if I shall through it away right the way because it is too old or may offer it to other departments. Sigma Aldrich guarantees only 1 year for this product but I guess it stays much longer in a good shape. Just HOW long? Does anyone have a personal experience?

Thank you for any response,
Paja

Hello,

I am looking for a video or detailed protocol (preferably video) for DRG (Dorsal Root Ganglia) extraction from adult mice, to culture dissociated DRG neurons. I found videos on Jove.com but unfortunately they are under neuroscience category to which my university doesn't have access. I am in desperate need for any help, and I would really appreciate it. Thank you for your time.

Best,
Maz