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| Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin |
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#1
03-30-2012, 02:14 PM
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 bisulphite conversion ratio Hi, I did a bisulphite conversion and via a cloning a sequencing afterwards. Not all the C are transformed into T. the conversion ratio should be more than 97% and I had to check it from my promoter. But do I have to calculate it for all the clones together or all seperately? thanks Kris  |
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#3
05-10-2012, 10:31 AM
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| Re: bisulphite conversion ratio In this case you can also find terms like H3K9me2 - and the 'me2' means dimethylation Sometimes shortcut 'ac' is also useful (it means acetylation) |
| The Following User Says Thank You to berton For This Useful Post: | ||
Marcoen (05-13-2012)
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| bisulphite , conversion , ratio |
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