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Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


bisulphite conversion ratio

bisulphite conversion ratio - Epigenetics Forum: DNA Methylation, Histone and Chromatin Study

bisulphite conversion ratio - An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


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Old 03-30-2012, 02:14 PM
Pipette Filler
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Default bisulphite conversion ratio



Hi,

I did a bisulphite conversion and via a cloning a sequencing afterwards.
Not all the C are transformed into T.

the conversion ratio should be more than 97% and I had to check it from my promoter.
But do I have to calculate it for all the clones together or all seperately?

thanks

Kris
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Old 04-26-2012, 03:41 AM
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Default Re: bisulphite conversion ratio

Hi

You can find this info by using search box in the top of website with some keywords related before posting questions.
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Old 05-10-2012, 10:31 AM
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Default Re: bisulphite conversion ratio

In this case you can also find terms like H3K9me2 - and the 'me2' means dimethylation Sometimes shortcut 'ac' is also useful (it means acetylation)
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