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-   -   bisulphite conversion ratio (http://www.molecularstation.com/forum/epigenetics-forum-dna-methylation-histone-chromatin-study/85037-bisulphite-conversion-ratio.html)

Marcoen 03-30-2012 02:14 PM

bisulphite conversion ratio
 
Hi,

I did a bisulphite conversion and via a cloning a sequencing afterwards.
Not all the C are transformed into T.

the conversion ratio should be more than 97% and I had to check it from my promoter.
But do I have to calculate it for all the clones together or all seperately?

thanks

Kris

hua052011 04-26-2012 03:41 AM

Re: bisulphite conversion ratio
 
Hi

You can find this info by using search box in the top of website with some keywords related before posting questions.

berton 05-10-2012 10:31 AM

Re: bisulphite conversion ratio
 
In this case you can also find terms like H3K9me2 - and the 'me2' means dimethylation Sometimes shortcut 'ac' is also useful (it means acetylation)


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