Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Epigenetics Forum: DNA Methylation, Histone and Chromatin Study
Register Search Today's Posts Mark Forums Read

Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


choice of tag for ChIP

choice of tag for ChIP - Epigenetics Forum: DNA Methylation, Histone and Chromatin Study

choice of tag for ChIP - An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-08-2012, 02:48 PM
Pipette Filler
Points: 2,490, Level: 32 Points: 2,490, Level: 32 Points: 2,490, Level: 32
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2008
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default choice of tag for ChIP



Hi everyone,

I'm trying to ChIP a TF, but the AB for it doesn't really work. I have a version of this TF tagged with either a HA or a Strep tag, which I want to overexpress and then ChIP. Does anyone of you have experiences with one of the tags? Or did you get better results with any other tag? 6-HIS?
What negative AB do you use? I get high background with rbIgG....


Thanks for your help!
Reply With Quote
  #2  
Old 08-16-2012, 04:14 PM
Graduate Student
Points: 1,231, Level: 20 Points: 1,231, Level: 20 Points: 1,231, Level: 20
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2011
Posts: 113
Thanks: 0
Thanked 16 Times in 13 Posts
Default Re: choice of tag for ChIP

GFP tag works excellent for ChIP-seq
Reply With Quote
Reply

Tags
chip , choice , tag


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Multiple choice questions _Luna_ Cell Biology and Cell Culture 6 02-22-2012 07:52 PM
Is Medical Technology (aka Clinical Lab Science) a good career choice? Ferasha Science Jobs Forum 2 06-18-2011 03:06 PM
difficult choice between ABI 3500 and Beckman GenomeLab genomer Lab Equipment Discussion and Reviews 0 12-23-2009 08:03 PM
Is Medical Technology (aka Clinical Lab Science) a good career choice? Ferasha Science Jobs Forum 0 06-17-2009 05:16 PM
choice of stable mammalian expression/knock-in patrickzhang76@gmail.com Protocols and Methods Forum 0 08-02-2007 11:41 PM


All times are GMT. The time now is 10:39 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11473 seconds with 16 queries