Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Epigenetics Forum: DNA Methylation, Histone and Chromatin Study
Register Search Today's Posts Mark Forums Read

Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


Artificial/Forceful methylation of a specific DNA sequence in humans-Is it possible?

Artificial/Forceful methylation of a specific DNA sequence in humans-Is it possible? - Epigenetics Forum: DNA Methylation, Histone and Chromatin Study

Artificial/Forceful methylation of a specific DNA sequence in humans-Is it possible? - An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-11-2012, 06:54 AM
Pipette Filler
Points: 13, Level: 1 Points: 13, Level: 1 Points: 13, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2012
Posts: 3
Thanks: 1
Thanked 0 Times in 0 Posts
Default Artificial/Forceful methylation of a specific DNA sequence in humans-Is it possible?



Hi people! This is my first time using this forum. I am currently a student in high school who is really interested in biology. I am enrolled in a class where the students research a branch of science and conduct experiments with their mentors.

I am researching epigenetics/telomeres. For this upcoming science competition, I have created a research proposal. However, I don't know if it is doable.
My question for you guys is: Is artificially/forcefully methylating a specific DNA sequence in humans possible? I have searched the Internet, and came across DNA methyltransferase, which includes DNMT1, as well as DAM methylase. Each of these enzymes is responsible for the addition of a methyl group to a specific nucleotide in a given gene sequence. Could these enzymes be used in any way to methylate a specific gene? If not, please enlighten me with techniques to accomplish this. The proposal for the competition is due soon and I also have midterms to worry about, so any information would take off a lot of stress.

Thank you all so much.
Reply With Quote
  #2  
Old 01-13-2012, 05:30 PM
Graduate Student
Points: 1,231, Level: 20 Points: 1,231, Level: 20 Points: 1,231, Level: 20
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2011
Posts: 113
Thanks: 0
Thanked 16 Times in 13 Posts
Default Re: Artificial/Forceful methylation of a specific DNA sequence in humans-Is it possib

Quote:
Originally Posted by studentt View Post
Hi people! This is my first time using this forum. I am currently a student in high school who is really interested in biology. I am enrolled in a class where the students research a branch of science and conduct experiments with their mentors.

I am researching epigenetics/telomeres. For this upcoming science competition, I have created a research proposal. However, I don't know if it is doable.
My question for you guys is: Is artificially/forcefully methylating a specific DNA sequence in humans possible? I have searched the Internet, and came across DNA methyltransferase, which includes DNMT1, as well as DAM methylase. Each of these enzymes is responsible for the addition of a methyl group to a specific nucleotide in a given gene sequence. Could these enzymes be used in any way to methylate a specific gene? If not, please enlighten me with techniques to accomplish this. The proposal for the competition is due soon and I also have midterms to worry about, so any information would take off a lot of stress.

Thank you all so much.
DAM has been used to artificially methylate eukaryotic genes by fusing it to transcription factors (the technique is called DAM-ID). Perhaps you could engineer designer zinc fingers or TALEs fused to a methyltransferase to target specific genes.
Reply With Quote
The Following 2 Users Say Thank You to mmorgan For This Useful Post:
admin (01-15-2012), studentt (01-14-2012)
  #3  
Old 05-10-2012, 10:30 AM
Pipette Filler
Points: 32, Level: 1 Points: 32, Level: 1 Points: 32, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2012
Posts: 5
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: Artificial/Forceful methylation of a specific DNA sequence in humans-Is it possib

In this case you can also find terms like H3K9me2 - and the 'me2' means dimethylation Sometimes shortcut 'ac' is also useful (it means acetylation)
Reply With Quote
Reply

Tags
artificial or forceful , dna , humansis , methylation , sequence , specific


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Is it possible to artificially/forcefully methylate a specific DNA sequence in humans studentt Biology Forum 0 01-10-2012 01:28 PM
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
A Human Cytome Project - an idea - Update 14 March 2005 Peter Van Osta Cell Biology and Cell Culture 0 03-14-2005 02:27 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 10:18 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.19160 seconds with 16 queries