Why not directly sequence bisulfite-converted DNA?

[tfcheng]

Hi ALL,

I have a question about studying methylated DNA sequence. After bisulfite conversion, why don't we just sequence the whole thing instead of doing PCR? won't sequencing tell us everything we need? thank you!

[ClultyBuh]

hahaha

[PCR]

Originally Posted by tfcheng

sequence the whole thing

Because sequencing the "whole thing" costs a shedload of $$$$$

[Drekketh]

Originally Posted by PCR

Because sequencing the "whole thing" costs a shedload of $$$$$

and apart from that, it is still impossible. Even methylated DNA is not a continuous thread of nucleotide bases readily available for sequencing.

[mmorgan]

Originally Posted by tfcheng

Hi ALL,

I have a question about studying methylated DNA sequence. After bisulfite conversion, why don't we just sequence the whole thing instead of doing PCR? won't sequencing tell us everything we need? thank you!

BGI in China has done this. [Only registered users see links. ]