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Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


Why not directly sequence bisulfite-converted DNA?

Why not directly sequence bisulfite-converted DNA? - Epigenetics Forum: DNA Methylation, Histone and Chromatin Study

Why not directly sequence bisulfite-converted DNA? - An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


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Old 04-01-2011, 05:35 PM
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Default Why not directly sequence bisulfite-converted DNA?



Hi ALL,

I have a question about studying methylated DNA sequence. After bisulfite conversion, why don't we just sequence the whole thing instead of doing PCR? won't sequencing tell us everything we need? thank you!
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Old 04-10-2011, 02:37 PM
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hahaha
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Old 06-26-2012, 03:43 AM
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Default Re: Why not directly sequence bisulfite-converted DNA?

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Originally Posted by tfcheng View Post
sequence the whole thing
Because sequencing the "whole thing" costs a shedload of $$$$$
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Old 06-26-2012, 09:41 AM
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Default Re: Why not directly sequence bisulfite-converted DNA?

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Originally Posted by PCR View Post
Because sequencing the "whole thing" costs a shedload of $$$$$
and apart from that, it is still impossible. Even methylated DNA is not a continuous thread of nucleotide bases readily available for sequencing.
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Old 08-16-2012, 04:18 PM
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Default Re: Why not directly sequence bisulfite-converted DNA?

Quote:
Originally Posted by tfcheng View Post
Hi ALL,

I have a question about studying methylated DNA sequence. After bisulfite conversion, why don't we just sequence the whole thing instead of doing PCR? won't sequencing tell us everything we need? thank you!
BGI in China has done this. [Only registered users see links. ]
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