Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Epigenetics Forum: DNA Methylation, Histone and Chromatin Study
Register Search Today's Posts Mark Forums Read

Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


Artificially made siRNAs

Artificially made siRNAs - Epigenetics Forum: DNA Methylation, Histone and Chromatin Study

Artificially made siRNAs - An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 10-19-2010, 12:44 PM
Pipette Filler
Points: 4, Level: 1 Points: 4, Level: 1 Points: 4, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2010
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Artificially made siRNAs



Hi,

I am currently attempting an experiment in which I deliver specific siRNAs into plant cells in order to silence a gene. I have the delivery medium. My only problem is that I don't where or how many siRNAs (says 21-22nt variety) to deliver in order to silence the gene.

I know the sequence of the gene to be silenced and also have a way of producing and amplifying up dsRNA (21-22nt) very easily.
(TROD siRNA design tool)

Would anyone be able to help?

Thanks,

McNeil
Reply With Quote
Reply

Tags
artificially , made , sirnas


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
siRNAs shRNAs microRNAs which to order? znsdavid RNAi and SiRNA Forum 0 05-20-2009 01:57 PM
Politics And Cannibalism? Introducing The Dourties, Chelsea, Bill, Hillary, Barrack Obama, George Bush, Jr., And All Of Capital Hill! jon_johnfrancisayres@yahoo.com Microbiology Forum 0 10-06-2007 05:59 AM


All times are GMT. The time now is 05:09 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13024 seconds with 16 queries