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| Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin |
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#1
10-19-2010, 12:44 PM
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 Artificially made siRNAs Hi, I am currently attempting an experiment in which I deliver specific siRNAs into plant cells in order to silence a gene. I have the delivery medium. My only problem is that I don't where or how many siRNAs (says 21-22nt variety) to deliver in order to silence the gene. I know the sequence of the gene to be silenced and also have a way of producing and amplifying up dsRNA (21-22nt) very easily. (TROD siRNA design tool) Would anyone be able to help? Thanks, McNeil  |
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