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|Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin|
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Artificially made siRNAs
I am currently attempting an experiment in which I deliver specific siRNAs into plant cells in order to silence a gene. I have the delivery medium. My only problem is that I don't where or how many siRNAs (says 21-22nt variety) to deliver in order to silence the gene.
I know the sequence of the gene to be silenced and also have a way of producing and amplifying up dsRNA (21-22nt) very easily.
(TROD siRNA design tool)
Would anyone be able to help?
|artificially , made , sirnas|
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