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| Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin |
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12-06-2006, 07:29 PM
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 Transcriptional gene silencing Hello Can any one tell me the strategies to design shRNA for Transcriptional gene silencing(TGS).Or can I follow same procedure as for PTGS. With regards Meenakumari.        |
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12-06-2006, 08:19 PM
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| Re: Transcriptional gene silencing Hey there Muthukausalya and welcome to the forums  Sigma has a plasmid kit that may help you http://www.sigmaaldrich.com/Area_of_...NA_Design.html also GeneLink has an shRNA design software on the web you may find useful: http://www.genelink.com/sirna/shRNAi.asp TRC also has some rules that you may want to double check after using the program (GeneLink) to check: http://www.broad.mit.edu/genome_bio/trc/rules.html |
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