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Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


Problems with amplification of bisulfite converted DNA

Problems with amplification of bisulfite converted DNA - Epigenetics Forum: DNA Methylation, Histone and Chromatin Study

Problems with amplification of bisulfite converted DNA - An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


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Old 07-01-2010, 06:08 PM
Pipette Filler
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Question Problems with amplification of bisulfite converted DNA



Hi
Im new in this field of DNA methylation and Im having some difficulties regarding the amplification of bisulfite converted DNA. I think that the problem is not my primers, since they work well with "normal" DNA. Can be due to the DNA polymerase used? Which polymerase should I use to amplify bisulfite converted DNA?

Thanks a lot

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