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Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


Histone lysine methylation

Histone lysine methylation - Epigenetics Forum: DNA Methylation, Histone and Chromatin Study

Histone lysine methylation - An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


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Old 01-26-2010, 08:50 PM
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Default Histone lysine methylation



Hello,
I'd like to study the chromatin state at a promoter. I'm planning to do a ChIP using histone H3 antibodies, then a Taqman quantification with probe specific of my promoter of interest.
I want to use H3me3K4 to check the open state, but I'm kinda lost between using either H3me3K9 or H3me3K27 for repressed chromatin. I didn't find any previous papers where the authors have used either of them at this particular promoter. Which one might be a better marker of repressed chromatin ?
Thanks for your help.
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