|Register||Search||Today's Posts||Mark Forums Read|
|Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin|
| ||LinkBack||Thread Tools||Display Modes|
Histone lysine methylation
I'd like to study the chromatin state at a promoter. I'm planning to do a ChIP using histone H3 antibodies, then a Taqman quantification with probe specific of my promoter of interest.
I want to use H3me3K4 to check the open state, but I'm kinda lost between using either H3me3K9 or H3me3K27 for repressed chromatin. I didn't find any previous papers where the authors have used either of them at this particular promoter. Which one might be a better marker of repressed chromatin ?
Thanks for your help.
|histone , lysine , methylation|
|Thread||Thread Starter||Forum||Replies||Last Post|
|I need any information about mutual influence DNA methylation and hormonal signals, m||venefica||DNA Techniques||0||02-10-2009 08:12 AM|
|UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and deve||admin||Science News and Views||0||08-30-2007 04:50 PM|
|Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation||admin||Science News and Views||0||08-30-2007 04:50 PM|
|CpG ISLAND METHYLATION QUESTION||bboctor||Epigenetics Forum: DNA Methylation, Histone and Chromatin Study||1||07-03-2007 11:20 AM|
|New Saccharomyces Sequences 04/07/04||SGD Sequences||Yeast Forum||0||04-10-2004 11:48 PM|