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| Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin |
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#1
11-25-2008, 06:04 PM
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 M.SssI PROTOCOL I have a question ...its about the M.SssI protocol- I wonna use 25 ug DNA and to use the same relative quantities of reagents (that is written in the recommended protocol of NEB web site), I wonna incubate it for 4 hours , add more reagents (as a boost) ,incubate for 4 hours more and then to stop the reaction. my que. is this - should I purify the DNA afterwards? or is it ok to use this DNA for bisulfite treatment without purification? Thank you very much   |
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