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Epigenetics Forum: DNA Methylation, Histone and Chromatin Study An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


M.SssI PROTOCOL

M.SssI PROTOCOL - Epigenetics Forum: DNA Methylation, Histone and Chromatin Study

M.SssI PROTOCOL - An Epigenetics Forum Dealing with DNA Methylation, the study of histones and chromatin


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Old 11-25-2008, 07:04 PM
Pipette Filler
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Default M.SssI PROTOCOL



I have a question ...its about the M.SssI protocol- I wonna use 25 ug DNA and to use the same relative quantities of reagents (that is written in the recommended protocol of NEB web site), I wonna incubate it for 4 hours , add more reagents (as a boost) ,incubate for 4 hours more and then to stop the reaction.
my que. is this - should I purify the DNA afterwards? or is it ok to use this DNA for bisulfite treatment without purification?
Thank you very much
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Old 04-28-2010, 12:43 PM
Pipette Filler
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Default Re: M.SssI PROTOCOL

Yes you purify the DNA afterwards.
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