bioremediation of xenobiotics

[shweta agrawal]

hi...

i hav to do experiment on decomposition of DDT,detergent like xenobiotics by fungi.... how it wud be determine that how much of that compound is being degraded...how it quantified...n how much its concentration should be taken...either in broth or in media....

[danfive]

Check out p 12-15---Detergent Properties in the reference below

Originally Posted by danfive

For more background you may want to read "A Guide to the Properties and Uses of Detergents in Biology and Biochemistry" by Srirama M. Bhari
--> [Only registered users see links. ]

Perhaps measuring properties like light scattering, surface tension, charge, or some of the detergent removal techniques will be of help.

[shweta]

hi..thanx for this literature but...i have to remediate it by ungi.....how i may procced it either in media or broth 2 c its conc...gradient..plz giv those protocol in which it may determine by the help of colorimeter or spectrophotometr....
plz reply quickly....
thankx....

[aftabac]

Hi
first you have to isolate the xenobiotic resistant fungi, so you bring the sample and analyze it by putting it in broth at different concetration and then streak the fungi on agar media that have different concentration of xenobiotics and then you select the range at which the fungi grow.
then you start your experiment in broth with different concentration of xenobiotic and find out the values at which your organism of interest survive.
you can check the concentration by spectrophotmeter and you have to desgin the assay according to your metal or compound.
it is important to remember that certain compund are adhere to surface of organism and remove from the media but other are bioaccumuated within the organism.
and you can measure both the absorbed amound of xenobiotic as well as the present in your broth.
it is better you study some reseach article that have worked on xenobiotic and their degradation or removal by fungi, then you will exectly get the idea about exeperimental scheme and then you will better design your experiment.
best of luck for you
regards
aftab

[shweta]

hi..
thnx..tell me which type of compound i may prefer...n plz giv me information about the sites or article from which i may tak more idea..bze till nw i searched the literature bt i hav nerver get the exactly knowlegde......plz help..
thanx alot...

[danfive]

Here's what looks to be a really good paper ---> [Only registered users see links. ]
Christian Haglund. Biodegradation of xenobiotic compounds by the white-rot fungus Trametes trogii.

Excerpt:
"3.6.3 Degradation of xenobiotics
The degradation of anthraquinone-blue by the fungi was measured by adding 50
ppm of the dye to the 25 ml erlenmeyer culture, and then at different times take new
samples of the supernatant and measure the absorbance at 600 nm. The absorbance
was measured after 10 minutes, 30 minutes, 2 hours, 4 hours, 8 hours and 24 hours.
This procedure was done with cultures of varying growth time.
The minimal inhibitory concentration of PCBs for Trametes trogii was found by
investigating the growth at solid malt-agar medium prepared with different
concentrations of a PCB-mixture.
In the PCB-degradation experiments Trametes trogii were grown for five days
and then either 50 ppm or 100 ppm sonificated PCB-mixture was added to the
culture. Both liquid malt medium and basal medium were used in the experiments.
After a total growth of either 12 or 24 days, the growth was stopped and the
cultures were prepared for analysis with gas-liquid chromatography. To stop the
growth and the enzymatic activity, the pH of the culture was lowered to around
pH=1.0 with 0.5 ml HClO4. As an internal control, 50 ppm of biphenyl dissolved in
hexane was added to every culture. In order to separate the remaining PCB from the
culture, 10 ml of hexane was added to each one. The cultures were then shaken and
stored overnight at 8C. The next day as much as possible of the hexane-phase was
separated from the cultures. To the hexane-phase, 2 g of Na2SO4 was added in
order to bind the remaining water. The hexane-phase was then stored at -20C until
the analysis with gas-liquid chromatography.
The chromatograph was a Hewlett-Packard, model 5840A, with a HP1
column (cross-linked methyl siloxane) 10 m length, 0.53 mm id. Detector: FID
(flame ionizer detector). Injector temperature: 200 ºC. FID temperarure 310 ºC.
Initial temperature (of column) 80 ºC, for 2 min. Rate 10 ºC/min. Final temperature
200 ºC, for 15 min."

[shweta]

hi...
thanx alot....
ill try to proceed.....

[danfive]

Originally Posted by shweta

hi...
thanx alot....
ill try to proceed.....

Lots of luck!!!

[edward81]

This experiment is very interesting, and I want know more abour this experiment. What is the process ? and What is the cost? and other detalls of this.

Lots of luck

Edward

[Jorge1907]

This string was real silly. Do you guys really think a person so obviously ignorant of literature cam pull off a meaningful experiment, esp with the limited info provided?