I was very happy to see that there is a forum for ELISA discussions.
I have a problem with plate drift. I add samples by single channel pipett from microtubes(1.5ml). I do this since we have very low sample volumes (220myliters) and I wish to mix samples carefully and doing this using racks and multichannel pipett I found difficult. The drift is not very strong but is clearly seen when runing standard in collumn 1 and column 12, a value of 2.3 at first column is typicaly 2.0 at the last. The time difference from adding first to last sample is 20-25minutes. The incubation time is then 120min at 37C
Is there a some way to reduce this drift, exept from being quicker to add samples. I was thinking that i could change the incubation time from 120min at 37C to 18H at 4C to allow a steady state to establis.
Would appreciate any help