Is total protein determination always necessary before an Elisa assay?
I want to measure a protein in Bronchoalveolara Lavage Fluid (BALf) which is the lining fluid from the bronchial and alveolar epithelium. It is aspirated with saline through Bronchoscope
What is the point of measuring the total protein concentration??
The most papers i have reviewed, using Elisa in BALf, have results in pg/ml or ng/ml or ug/ml and they have not performed an assay for the total protein concentration.
Thanks in advance for the replies