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#1
11-24-2012, 12:45 PM
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 Sample dilution Hi everyone, I'm a newbie with ELISA and I have a basic question. I'm working with mouse serum in order to quantify IFN-gamma and my supervisor asked me to dilute 2x the sample in assay diluent. So once I get my OD, in order to calculate the original concentration : Do I have to multiply the OD by 2, or do I have to multiply the calculated concentration by 2? Thank you for your help Dusan  |
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