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|ELISA Assay Forum ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.|
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I'm a newbie with ELISA and I have a basic question.
I'm working with mouse serum in order to quantify IFN-gamma and my supervisor asked me to dilute 2x the sample in assay diluent.
So once I get my OD, in order to calculate the original concentration :
Do I have to multiply the OD by 2, or do I have to multiply the calculated concentration by 2?
Thank you for your help
Re: Sample dilution
multiply the calculated concentration off the standard curve by 2. Generally you dilute so that it will be in the OD range for the standard curve
|dilution , sample|
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