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Sample dilution

Sample dilution - ELISA Assay Forum

Sample dilution - ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


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Old 11-24-2012, 01:45 PM
Pipette Filler
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Default Sample dilution



Hi everyone,

I'm a newbie with ELISA and I have a basic question.

I'm working with mouse serum in order to quantify IFN-gamma and my supervisor asked me to dilute 2x the sample in assay diluent.

So once I get my OD, in order to calculate the original concentration :

Do I have to multiply the OD by 2, or do I have to multiply the calculated concentration by 2?

Thank you for your help

Dusan
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Old 11-26-2012, 02:26 PM
Pipette Filler
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Default Re: Sample dilution

multiply the calculated concentration off the standard curve by 2. Generally you dilute so that it will be in the OD range for the standard curve
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