I'm a newbie with ELISA and I have a basic question.
I'm working with mouse serum in order to quantify IFN-gamma and my supervisor asked me to dilute 2x the sample in assay diluent.
So once I get my OD, in order to calculate the original concentration :
Do I have to multiply the OD by 2, or do I have to multiply the calculated concentration by 2?
Thank you for your help
Re: Sample dilution
multiply the calculated concentration off the standard curve by 2. Generally you dilute so that it will be in the OD range for the standard curve
|All times are GMT. The time now is 01:09 PM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved