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casein as a block

casein as a block - ELISA Assay Forum

casein as a block - ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


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  #1  
Old 07-09-2012, 10:22 AM
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Default casein as a block



Hi

does anyone have a foolproof recipe for preparing a casein blocking buffer for ELISA - my old one no longer seems to work:

0.5% casein in PBS, heat to dissolve - still not dissolved!

Using Sigma C5890 casein.

thanks
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Old 07-09-2012, 09:46 PM
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Default Re: casein as a block

If you are set on using the same product from Sigma, then you may have to increase the pH and then adjust back once it's into solution.

I have used I Block (Tropix/applied biosystems) which goes into solution at 90 degrees as you descripbe and is excellent for many assays, or alternatively use pre-prepared Hammarsten grade casein from pierce.

I suspect the sigma stuff might be of lesser and perhaps variable purity affecting solubility

good luck
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Old 08-01-2012, 01:09 PM
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Default Re: casein as a block

Thanks

I have since bought in some pre-prepared 1% Casein from Thermo Pierce which works a treat.
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Old 09-04-2012, 04:06 PM
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Default Re: casein as a block

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