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#1
04-23-2012, 10:43 PM
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 Recommended maximum OD values in ELISA optimization. What's the reason? Greetings to all, ye Lore Keepers. Ths is my first post.  I'm a postgraduate student in Argentina.I'm optimizing an indirect competitive ELISA to quantify a small molecule in plant tissue. Reading Crowther's ELISA Guidebook (awfully edited) i've found that when optimizing a competitive ELISA, the recommended antigen concentration to coat plates with is that which results in a maximum OD (plateau) of 1-1.5 for antibody excess. And an antibody dilution which results in 70% of that maximum OD (since competitive ELISAs are reagent limited). My question is: why not greater OD plateau values e.g. 2.0 (provided we are having the same background OD)? I'm using a Tecan Sunrise plate reader. Any ideas? Thanks in advance!  |
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| competitive elisa , elisa , maximum , od values , optimization , reason , recommended , values |
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