Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > ELISA Assay Forum
Register Search Today's Posts Mark Forums Read

ELISA Assay Forum ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


Reducing the concentration required for the linear range of the assay

Reducing the concentration required for the linear range of the assay - ELISA Assay Forum

Reducing the concentration required for the linear range of the assay - ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-08-2012, 06:10 PM
Pipette Filler
Points: 490, Level: 9 Points: 490, Level: 9 Points: 490, Level: 9
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2011
Posts: 4
Thanks: 0
Thanked 0 Times in 0 Posts
Default Reducing the concentration required for the linear range of the assay



Hi,

We are using a kit to detect a range of cytokines using ELISA. The linear range for these cytokines is significantly higher than the concentration of the protein in our samples.

We would consider using more protein, with less dilution; however, our sample protein is a very limited resource, and this is impossible.

Is it possible to reduce the concentration of antigen at which the linear range shows itself--that is, shift the curve to the left--using some manipulation of reading equipment? Or is this only possible by changing antibody concentrations (i.e. not using a kit and developing the assay in-house) or incubation times? Are there any other potential workarounds for this problem?

Thank you,
Kevin
Reply With Quote
Reply

Tags
assay , concentration , linear , range , reducing , required


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
New Saccharomyces Sequences 11/27/04 Mike Cherry Yeast Forum 0 11-29-2004 12:39 AM
New Saccharomyces Sequences 09/08/04 SGD Sequences Yeast Forum 0 09-13-2004 10:07 PM
New Saccharomyces Sequences 08/11/04 SGD Sequences Yeast Forum 0 08-12-2004 12:26 AM
New Saccharomyces Sequences 05/19/04 SGD Sequences Yeast Forum 0 05-23-2004 04:06 PM


All times are GMT. The time now is 07:31 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13505 seconds with 16 queries