I'm doing research that involves infusing an animal with 1 ml of 43mM melatonin solution and then analyzing its serum with a melatonin(MT) ELISA that has a standard range of 12.ng/ml-400ng/ml. The solution will be diluted by the animals blood, which has mean volume of 11.6ml but I'm concerned that the MT concentration form the serum samples will not fall into the standard range of the ELISA. I tried emailing the manufacturers to get an explanation:
"The concentration of the melatonin(MT) in the mouse immediate after infusion is about 3385.8 uM, with MW of MT at 232 dalton. The mass concnetration is 785.5 ug/ml. If you do 2-fold serial dilution in a 96-well plate into PBS or your buffer, the 9th dilution will give you 383 ng/mL, 10th dilution 192 ng/mL, and 11th dilution 96 ng/mL, 12th dilution 48 ng/ml. All 9th to 12th dilution fall into the detection range."
I don't understand how they calculated 3385.8uM from a 43mM MT infusion. I tried doing my own calculations but I'm always off by a few thousand uM. If someone could please dumb down this explanation I would appreciate it greatly!!