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Kit Supplied Standards reading Incorrectly

Kit Supplied Standards reading Incorrectly - ELISA Assay Forum

Kit Supplied Standards reading Incorrectly - ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


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Old 03-23-2011, 08:19 AM
Pipette Filler
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Exclamation Kit Supplied Standards reading Incorrectly



Hi all,

I am new to the world of ELISA and I am having some trouble getting my standard curve to work.

I purchased a competitive ELISA kit to measure Salivary Cortisol in humans.

So far I have run 4 trials but none of them have produced readings to match the concentrations of the 6 standards I recieved in the kit.

For example - One of the standards is supposed to contain 3.0 ug/dL however the absorbance readings lead to a calculated concentration of only 2.551 ug/dL

Does anyone know why this would continue to happen? My duplicate wells match almsot perfectly, I centrifuge and vortex my controls and standards as required but continue to get incorrect results...

Any suggestions you could make would be much appreciated.

Thankyou, Jayde.
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elisa , incorrectly , kit , reading , salivary cortisol , standard curve , standards , supplied , trouble


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