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ELISA Assay Forum ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


Colour all over

Colour all over - ELISA Assay Forum

Colour all over - ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


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Old 03-19-2011, 08:08 AM
Pipette Filler
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Default Colour all over



Hey
I am doing the human sRANKL ELISA (peprotech), and I get colour all over the plate, ie the same colour in the blank as well as all the standards and the samples as well!

I tried blocking with FCS 10% instead of 1% BSA, that just reduced the ODs, the colour still remained similar.
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