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|ELISA Assay Forum ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.|
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Colour all over
I am doing the human sRANKL ELISA (peprotech), and I get colour all over the plate, ie the same colour in the blank as well as all the standards and the samples as well!
I tried blocking with FCS 10% instead of 1% BSA, that just reduced the ODs, the colour still remained similar.
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