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|ELISA Assay Forum ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.|
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Relative quantitative ELISA
I'm doing ELISA additional to RT-qPCR. I'm interested in relation between change in protein expression level to protein quantity increase. For ELISA I got to determine the relative protein change after cell stimuli. So I need a reference. Is it better to use complete cell protein as reference object, or can I use protein amount measured by ELISA from starved medium from cells (bevore stimuli addition) ?
Thanks for helping me.
Last edited by Omicron Lyrae; 10-05-2010 at 06:34 AM. Reason: Base question is solved
|bevor , cell , cell culture , culture , elisa , free , lysis buffer , media , reference , serum|
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