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ELISA Assay Forum ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


Serum free media for cell culture bevor ELISA

Serum free media for cell culture bevor ELISA - ELISA Assay Forum

Serum free media for cell culture bevor ELISA - ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


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Old 10-03-2010, 08:08 AM
Pipette Filler
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Question Relative quantitative ELISA



I'm doing ELISA additional to RT-qPCR. I'm interested in relation between change in protein expression level to protein quantity increase. For ELISA I got to determine the relative protein change after cell stimuli. So I need a reference. Is it better to use complete cell protein as reference object, or can I use protein amount measured by ELISA from starved medium from cells (bevore stimuli addition) ?

Thanks for helping me.

Last edited by Omicron Lyrae; 10-05-2010 at 06:34 AM. Reason: Base question is solved
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bevor , cell , cell culture , culture , elisa , free , lysis buffer , media , reference , serum


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