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Store ELISA plate

Store ELISA plate - ELISA Assay Forum

Store ELISA plate - ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


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Old 10-27-2009, 07:32 PM
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Smile Store ELISA plate



Hi everyone!
If I want to store an ELISA plate to do the assay another day, in which step can I do it, and for how long time could be store??? I mean:
- to store the plate with the antigen. Wash or not. which temperature.
- with blocking buffer. Wash or not. which temperature.
I really appreciate your answers.
Regards,
freyes.
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Old 10-27-2009, 08:42 PM
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Default Re: Store ELISA plate

Several ELISA development manuals say to store the ELISA plate dry after your blocking step.
You can also store it with Wash buffer, ON, I've done this.
Both at 4C.
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Old 01-14-2013, 09:13 PM
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Default Re: Store ELISA plate

I almost always pre-coat multiple (20+) ELISA microplates and store them at 4deg C for 1-2 months. I coat the plates with antigen, incube, wash, block and then aspirate. After aspirating the plates, I let them dry upside down on a bench pad and then I put them into Mylar bags (1 plate per bag) with a desiccant pouch in each bag.

I have run ELISAs comparing stored vs. freshly coated plates and have not seen any variability between the two.
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