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|ELISA Assay Forum ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.|
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In my new lab they use skimmed milk to block ELISA plates even for the detection of bovine antibodies. I say that this is not a good idea. What do you think?
Re: blocking reagents
I don't think so.Although the milk include IgG of bovine,BSA bind to the solid phase.BSA is not a good antigen to animal.
I think is not a problem to it.Is the back-ground color high?
|blocking , reagents|
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