blocking reagents

bgt · #1 05-07-2009, 08:51 PM

In my new lab they use skimmed milk to block ELISA plates even for the detection of bovine antibodies. I say that this is not a good idea. What do you think?

ljpyou · #2 05-14-2009, 02:04 PM

I don't think so.Although the milk include IgG of bovine,BSA bind to the solid phase.BSA is not a good antigen to animal.
I think is not a problem to it.Is the back-ground color high?

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blocking reagents

blocking reagents - ELISA Assay Forum

blocking reagents - ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.